rabbit polyclonal antibody to e2f4 (Danaher Inc)
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rabbit polyclonal antibody to e2f4
Rabbit Polyclonal Antibody To E2f4, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 20243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+to+e2f4/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc07146881-104-9-17
Average 99 stars, based on 20243 article reviews
Rabbit Polyclonal Antibody To E2f4, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 20243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+to+e2f4/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc07146881-104-9-17
Average 99 stars, based on 20243 article reviews
rabbit polyclonal antibody to e2f4 - by Bioz Stars,
2026-10
99/100 stars
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1) Product Images from "Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation"
Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation
Journal: Cancer Cell International
doi: 10.1186/s12935-020-01167-1
Figure Legend Snippet: Overexpression of GAS5 prevents H3K27 trimethylation and upregulates CDKN1C expression to accelerate oxidative stress in melanoma by inhibiting EZH2. a Correlation analysis of lncRNA GAS5 expression and EZH2 expression. b RIP assay of the recruitment of transcription factor E2F4 by lncRNA GAS5. c ChIP assay of the enrichment of E2F4 in EZH2 promoter region. d RNA pull-down assay of the direct interaction of lncRNA GAS5 and E2F4. A375 cells were treated with oe-GAS5 alone or in the presence of oe-EZH2. e RT-qPCR assay of lncRNA GAS5 expression and mRNA expression of EZH2 and CDKN1C. f Western blot assay of the protein expression of E2F4, EZH2, H3K27me3 and CDKN1C. g Western blot assay of the protein expression of MDA5, IRE1α and SOD-1. h ELISA analysis of the content of ROS. i CCK-8 assay of cell viability. j Flow cytometric analysis of cell apoptosis. * p < 0.05, compared with the IgG group, cells transduced with oe-NC, Bio-probe NC group, cells transduced with oe-G-NC or cells transduced with oe-GAS5 + oe-E-NC. The above measurement data are expressed as mean ± standard deviation. The unpaired t -test is used for comparison of the data between two groups. Data among multiple groups are analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The statistical analysis concerning time-based measurements within each group is realized using ANOVA of repeated measurements, followed by a Bonferroni’s post hoc test. Pearson correlation analysis is performed for correlation analysis
Techniques Used: Over Expression, Expressing, Pull Down Assay, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Transduction, Standard Deviation, Comparison
Related Articles
Over Expression:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Expressing:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Pull Down Assay:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Quantitative RT-PCR:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Western Blot:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Enzyme-linked Immunosorbent Assay:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary CCK-8 Assay:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Transduction:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Standard Deviation:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary Comparison:Article Title: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation Article Snippet: The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.The sample and input were detached with proteinase K to extract RNA for subsequent western blot assay.. The antibodies were recruited for RIP including the primary |
